Fig. 7
m6A modification is associated with LINC00958 upregulation in HCC cells. a m6A RIP-qPCR analysis showed that m6A was highly enriched within LINC00958 in HCC cells. b RT-qPCR was performed to verify the knockdown efficiency of METTL3 in HCCLM3 and Focus cells. c Western blotting was used to confirm the knockdown efficiency of METTL3 in HCCLM3 and Focus cells. d The m6A level of LINC00958 was examined in HCCLM3 and Focus cells with METTL3 knockdown. e The expression level of LINC00958 was assessed in HCCLM3 and Focus cells with METTL3 knockdown. f HCCLM3 cells with METTL3 knockdown were treated with actinomycin D for the indicated time points, and the expression level of LINC00958 was examined using RT-qPCR. g Focus cells with METTL3 knockdown were treated with actinomycin D for the indicated time points, and the expression level of LINC00958 was examined using RT-qPCR. h HCCLM3 and Focus cells were treated with or without 5-aza-dC, and LINC00958 expression level was examined using RT-qPCR. i HCCLM3 and Focus cells were treated with specific inhibitors of HDAC1 (PCI-24781), HDAC3 (RGFP966), HDAC6 (ACY-1215), and broad-spectrum HDAC inhibitor (SAHA). RT-qPCR was performed to examine the expression levels of LINC00958. j Schematic diagram demonstrating the molecular mechanisms underlying LINC00958 in HCC