Fig. 7

AGK activates the PI3K/AKT/GSK3β signalling pathway and β-catenin nuclear translocation in RCC cells. a Representative images of human phosphor-kinase array analysis. b Statistical analysis of the relative levels of the phosphorylation of 43 kinase phosphorylation sites in the indicated RCC cells. c The expression of p-GSK-3a and p-GSK-3β was detected by Western blot analysis. d Western blot analysis of the expression of p-AKT, p-PI3K and p-GSK-3β in cells transfected with a recombinant lentivirus carrying a human AGK overexpression plasmid. GAPDH served as the loading control. e The cellular location of β-catenin was examined by immunofluorescence staining. f Luciferase assay analysis of the transcriptional activity of TCF/LEF in the indicated cells. *P < 0.05; **P < 0.01