Fig. 6

HADHA promotes β-oxidation of fatty acids in HCC. a OCR, the activities of β-oxidation, in HepG2 cells treated with 100 μM ETO, as well as in HepG2^miR-612-KD, HepG2^hadha-OE, and HepG2^NC cells. b OCR, the activities of β-oxidation, in HCCLM3 cells treated with 100 μM ETO, as well as in HCCLM3^miR-612-OE, HCCLM3^hadha-KD, and HCCLM3^NC cells. c The protein levels of Cortactin in HCCLM3 cells treated with 100 μM ETO or 100 mM linoleic acid, as well as in HCCLM3^miR-612-o, HCCLM3^miR-612-i, HCCLM3^hadha-o, and HCCLM3^hadha-i cells. d The intracellular levels of acetyl CoA in HCCLM3^miR-612-o, HCCLM3^hadha-i, and HCCLM3^NC cells. e The cellular levels of cholesterol in HepG2^miR-612-KD, HCCLM3^miR-612-OE, HepG2^hadha-OE, and HCCLM3^hadha-KD cells. f The protein levels of Cortactin, Caveolin-1, E-cadherin, and GAPDH in HCCLM3 cells treated with or without 1 mM MβCD. g Fluorescence intensity in HepG2^miR-612-i, HCCLM3^miR-612-o, HepG2^hadha-o, and HCCLM3^hadha-i cells detected by fluorescence spectrophotometer (Ex/Em = 360/460 nm). Fluorescence polarization is calculated by the formula (h) The levels of ATP in HepG2^miR-612-KD, HCCLM3^miR-612-OE, HepG2^hadha-OE, and HCCLM3^hadha-KD cells analyzed by spectrophotometer (λ = 636 nm). (NS: no significance; *p < 0.05; **p < 0.01; ***p < 0.001). Data are mean ± SEM of three independent experiments