Fig. 7
From: MiR-612 regulates invadopodia of hepatocellular carcinoma by HADHA-mediated lipid reprogramming
Clinical significance of HADHA in HCC patients. a The cholesterol and 27-hydroxycholesterol of HepG2 and HCCLM3 cells relative quantification based on liquid chromatography mass spectrometry (LC-MS)/MS system. (NS: no significance; **p < 0.01; ***p < 0.001). b Immunoblotting of proteins involved in cholesterol-biosynthesis pathway in HepG2hadha-OE, HCCLM3hadha-KD cell, and their negative control cells. (Cells treated with negative control lentivirus). c Negative correlation between miR-612 and hadha mRNA in 15 HCC and their adjacent normal tissues. d Representative images of negative and positive HADHA staining in tumor and peritumor tissue from one HCC patient. Bars: (left) magnification × 100, scale bars, 500 μm (right) magnification × 400, scale bars, 50 μm. e The average densities of HADHA in 134 HCC and their paired normal tissues. f, g Kaplan-Meier analyses of PFS and OS in HCC patients using SPSS 22.0. h Hypothesis diagram of lipid reprogramming in HCC cells modulated by miR-612/HADHA/cholesterol axis