Fig. 3
From: The design and development of covalent protein-protein interaction inhibitors for cancer treatment
The general workflow of the isoTOP-ABPP platform. The cell proteome is labeled with a cysteine-reactive probe, and then samples are conjugated to TEV protease-cleavable biotin tags (heavy (blue) and light (red)). Competition ratios are calculated following protein digestion and multidimensional liquid chromatography-tandem mass spectrometry