Fig. 2

HUMT regulates lymph node metastasis of triple-negative breast cancer cells in vitro. a HUMT expression in breast cancer cell lines. MCF10A represents normal breast epithelial cells. b HUMT was more highly expressed in the basal-like subtype in a comprehensive analysis of the GEO datasets and TCGA database. c Representative graphs (left) and quantification (right) of wound healing assay. Representative graphs (left) and quantification (right) of Transwell migration (d) and invasion e assay in modulated MDA-MB-231 and BT549 cells. f Representative graphs of 3D invasion assay in modulated MDA-MB-231 and BT549 cells. g Representative picture (left) and quantification (right) of HLEC migration assay. h Representative graphs (left) and branch number and total tube length quantification (right) of HLEC tube formation assay cultured with medium supernatant from NC or HUMT-KO-modulated MDA-MB-231 and BT549 cells. Data were shown as mean ± SD; **P < 0.01