Fig. 3

HUMT expression was regulated in an epigenetic way. a Bioinformatic analysis indicated CpG enrichment in the promoter region, and patients with basal-like subtype exhibited a lower HUMT methylation level. b HUMT expression was significantly correlated with a methylation probe signal located in the CpG island of the promoter region. c, d Overall DNA methylation level of HUMT was significantly correlated with RNA expression in patients with breast cancer and TNBC of the TCGA database. e, f Overall DNA methylation level of HUMT was significantly correlated with RNA expression in all cell lines and breast cancer cell lines of the CCLE database. g Representative hypermethylation status in BT483, MCF-7, and ZR-75-1 cells (CCLE). h A methyltransferase inhibitor, DAC, could significantly upregulate the expression level of HUMT in BT483, ZR-75-1, and MCF-7 cells. Data were shown as mean ± SD; *P < 0.05; **P < 0.01; **P < 0.001