Fig. 2

Internalization capacity and antineoplastic activity of T22-GFP-H6 and T22-GFP-H6-Auristatin in AML cell lines. a CXCR4 membrane expression of THP-1 and SKM-1 AML cell lines by flow cytometry. b Evaluation of T22-GFP-H6-Auristatin internalization, by flow cytometry, in CXCR4+ leukemic cells after 1 h of incubation at different concentrations. c Competition assay with AMD3100 (240 nM T22-GFP-H6-Auristatin and 2000 nM AMD3100) to determine the specificity of internalization through CXCR4 receptor. d Evaluation of antineoplastic activity of T22-GFP-H6 after 48 h of treatment in AML cell lines performed by XTT assay. e Anticancer activity of T22-GFP-H6-Auristatin in AML cell lines after 48 h of incubation by XTT assay. f Competition of antineoplastic activity of T22-GFP-H6-Auristatin at 240 nM with AMD3100 at 2000 nM after 48 h exposure by XTT assay. Results are presented as mean ± SE MFI in a, b, and c, and mean ± SE percent of cell viability in d, e, and f. U of Mann-Whitney test was used to test differences between groups. In b and e, T22-GFP-H6-Auristatin treated cells were compared with the buffer treated cells (Ctrl). Statistical significant differences are indicated by * when p value < 0.05 and ** when p value < 0.001. n.s. means no significance. AML, acute myeloid leukemia; Ctrl, control; MFI, mean fluorescence intensity; SE, standard error