Fig. 3

Study of cell death in AML cell lines caused by T22-GFP-H6-Auristatin treatment. Study of the mechanism of T22-GFP-H6-Auristatin-induced cell death. a Induction of apoptosis and mitotic catastrophe by T22-GFP-H6-Auristatin at 240 nM for 24 and 48 h of exposure detected by DAPI staining in CXCR4-positive THP-1 and SKM-1 leukemic cell lines. Yellow and red arrows point to MC figures and apoptotic bodies, respectively. b Quantitation of the number of cells undergoing mitotic catastrophe triggered by treatment with T22-GFP-H6-Auristatin at 240 nM in THP-1 and SKM-1 for 24 (orange bars) or 48 h (yellow bars). c Quantitation of the number of apoptotic bodies in THP-1 and SKM-1 induced by exposure to 240 nM T22-GFP-H6-Auristatin for 24 (orange bars) or 48 h (yellow bars). Results in b and c are presented as mean ± SE cell number for each cell death event (MC or apoptosis) in all fields analyzed. * indicates significant differences between groups with a p value < 0.05. CTRL, control; MC, mitotic catastrophe; SE, standard error