Fig. 5

Antineoplastic activity of T22-GFP-H6-Auristatin in a disseminated AML mouse model. a Experimental design of the in vivo assay used to assess the antineoplastic activity of T22-GFP-H6-Auristatin. b Measurement of total body weight of mice during the experimental time according to the treatment group. Results are presented as mean ± SE body weight in grams. c Comparison of evolution of bioluminescence emission in mice treated with vehicle (VEH) or T22-GFP-H6-Auristatin (T22-AUR) and untreated healthy mice (Normal group) the days 4, 8, 11, and 13 after injection of THP-1-Luci cells, measured by the IVIS Spectrum. d Follow-up of total body bioluminescence emission of mice treated with T22-GFP-H6-Auristatin (T22-AUR) or vehicle (VEH) and untreated healthy mice (Normal) during all the experiment, analyzed in IVIS Spectrum. Results are presented as mean ± SE luminescence values in photons per second (total flux [p/s]). U of Mann-Whitney test was used to assess significant differences between groups in these studies (b, d), and these differences were considered statistically significant when the p value was lower than 0.05. * indicates differences between the VEH and T22-AUR groups, # between the Normal and VEH groups, and ‡ between the Normal and T22-AUR groups. BLI, bioluminescence; T22-AUR, T22-GFP-H6-Auristatin group; VEH, vehicle group.;SE, standard error