Skip to main content
Fig. 7 | Journal of Hematology & Oncology

Fig. 7

From: An Auristatin nanoconjugate targeting CXCR4+ leukemic cells blocks acute myeloid leukemia dissemination

Fig. 7

Detection of CD45-positive cells in the leukemia-infiltrated tissues after treatment with T22-GFP-H6-Auristatin. a Detection of CD45-positive cells by IHC in the bone marrow and extramedullar organs (spleen and liver) of mice treated with vehicle (VEH) or T22-GFP-H6-Auristatin (T22-AUR) 14 days after injection of CXCR4+ THP-1-Luci cells, and comparison with CD45-positive cell detection in same tissues of a normal non-leukemic mouse. b Quantification of the CD45 detection by IHC in the spleen, liver, and bone marrow in mice treated with vehicle (VEH, blue bars) and T22-GFP-H6-Auristatin (T22-AUR, orange bars). This quantification is presented as mean ± SE percentage of stained surface with CD45 antibody in the tissue of all mice. c Quantitation of CD45 staining intensity by IHC in the liver, spleen, and bone marrow in mice treated with vehicle (VEH, blue bars) or T22-GFP-H6-Auristatin (T22-AUR, orange bars). Results are presented as the mean ± SE of the mean gray value obtained in Image J (see the “Methods” section). Significant differences were indicated by * when the p value was lower than 0.05, using U of Mann-Whitney test between groups. T22-AUR, T22-GFP-H6-Auristatin group; VEH, vehicle group; SE, standard error

Back to article page