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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: CRISPR/CAS9-mediated knockout of Abi1 inhibits p185Bcr-Abl-induced leukemogenesis and signal transduction to ERK and PI3K/Akt pathways

Fig. 1

Generation of Abi1 deficient cell lines in p185Bcr-Abl-transformed BaF3 cells. a. Sequencing analysis of indel mutations in p185Bcr-Abl Abi1 knockout cells. Sequences targeted by gRNA A and B are underlined and the PAM sequences are in red. b. Abi1 expression in Ba/F3, p185 control (Cas9 ctrl), and two independent p185Bcr-Abl Abi1 knockout cell lines, KO2.3 and KO6.2. c. Abi2 expression in Ba/F3, Cas9 ctrl, KO2.3 and KO6.2 cells. Total lysates from 5 × 105 cells of each cell line, as indicated, were subjected to Western blot analysis with indicated antibodies

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