CRISPR/CAS9-mediated knockout of Abi1 inhibits p185Bcr-Abl-induced leukemogenesis and signal transduction to ERK and PI3K/Akt pathways

Table 1 Summary of the disease development in mice injected with p185 control cells and the p185 KO2.3 cells

Mouse	Latency ^a(days)	Spleen weight (g)	Liver weight (g)
Saline Ctrl
A1	60^b	0.07	1.14
A2	125^b	0.08	1.30
A3	125^b	0.08	1.36
A4	181^b	0.10	1.30
A5	181^b	0.09	1.27
p185 Cas9
A1	19^c	0.53	2.99
A2	20^c	0.74	2.77
A3	21^c	0.74	1.86
A4	23^c	1.05	1.49
A5	21^c	0.98	1.48
B1	15^c	0.44	1.88
B2	16^c	0.47	2.01
B3	16^c	0.39	1.28
p185 KO2.3
A1	43^d,e	0.09	1.1
A2	157^c,e	0.10	0.77
A3	181^b	0.11	1.33
A4	181^b	0.12	1.41
A5	181^b	0.14	1.73
B1	102^b	0.08	1.08
B2	116^c	0.40	0.94
B3	184^b	0.10	1.22
B4	184^b	0.11	1.26
B5	184^b	0.11	1.27

^aLatency is defined as the time post-injection that mice died or become moribund
^bThe day euthanized without any sign of disease
^cThe mice found moribund at the day of pathology analysis
^dThe mouse found dead at the day of pathology analysis
^eTumors were found around gastrointestinal tissue (A1) and in chest (A2) of mice

ISSN: 1756-8722