Fig. 1
From: Ubiquitination of the DNA-damage checkpoint kinase CHK1 by TRAF4 is required for CHK1 activation
CHK1 interacts with TRAF4. a Scatterplot showing the proteins pulled down by CHK1 and Control IgG antibodies. b Co-immunoprecipitation (Co-IP) analysis of the TRAF4 and CHK1 interaction in 293T cells transfected with the indicated constructs. WCE, whole-cell extract. c Co-IP analysis of the endogenous TRAF4-CHK1 interaction in HT29 cells. d Cell-free binding between TRAF4 and CHK1. e, f Schematic of CHK1 WT and deletion mutants (e) and TRAF4 WT and deletion mutants (f) used in this study. N, N-terminal; C, C-terminal; RING, RING domain; Intermediate, zinc finger and coiled-coil domain; TRAF, TRAF-C domain. g Co-IP analysis of TRAF4 and CHK1 truncation mutant interactions. 293T cells were transfected with the indicated constructs and subjected to Co-IP and immunoblotting (IB) analysis. h Co-IP analysis of CHK1 and TRAF4 truncation mutant interactions. 293T cells were transfected with the indicated constructs and subjected to Co-IP and IB analysis. *Non-specific signal