Fig. 6
From: Ubiquitination of the DNA-damage checkpoint kinase CHK1 by TRAF4 is required for CHK1 activation
CHK1 phosphorylation positively correlates with TRAF4 overexpression in CRC patient samples. a Representative images of IHC staining for Ki67, TRAF4, p-CHK1 (S345), and CHK1 from 92 cases of primary tumors with matched adjacent tissues. b, c IHC scores for TRAF4 (b) and p-CHK1 (c) in 92 paired tumors and adjacent tissues. ***p < 0.001. d Kaplan–Meier curve shows the relationship between overall survival and TRAF4 expression in CRC patients. Data were obtained from the following online tool: https://hgserver1.amc.nl/cgi-bin/r2/main.cgi. e Representative images of IHC staining from a CRC primary tumor with high TRAF4 and p-CHK1 (S345) protein levels of or low TRAF4 and p-CHK1 (S345) protein levels. f The expression of TRAF4 and p-CHK1 (S345) is positively correlated. Pearson’s coefficient tests were performed to assess statistical significance. g The percentage of tissues displaying low or high TRAF4 and p-CHK1 (S345). ***p < 0.001. h Representative images of relapse tissues analyzed by IHC staining for Ki67, TRAF4, p-CHK1 (S345), and CHK1. i, j IHC scores of TRAF4 (i) and p-CHK1 (S345) (j) in paired primary and relapse samples. ***p < 0.001. k The expression of TRAF4 and p-CHK1 (S345) positively correlates in relapsed CRC tissues. Pearson’s coefficient tests were performed to assess statistical significance. l The percentage of specimens at initial diagnosis (left) and relapse (right) that display low or high TRAF4 and p-CHK1 (S345) levels after combination treatment. ***p < 0.001