Fig. 3

Macrophages stimulated by various tumor conditioned media affect the migratory ability of lung cancer cells. Transwell migration assay of A549 cells in response to THP-1 cells that had been treated with various conditioned media of A549 derivatives. A549 cells seeded in the upper chamber of transwells with inserts containing 8-μm membrane filters were cocultured with PMA-stimulated THP-1 cells, which had been treated with various conditioned media collected from A549-Oct4 cells (a), Oct4 knockdown A549 cells (b), or A549-Oct4 cells in the presence of anti-M-CSF antibody or control IgG (c), seeded in the lower chamber. After 6 h, A549 cells that migrated through the membrane to the lower surface were stained and quantified. Data shown represent means ± SEM (n = 3 in a, b; n = 10 to 12 in c). Data shown represent mean ± SEM. Results are representative of two independent experiments