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Fig 1 | Journal of Hematology & Oncology

Fig 1

From: Th17 cells inhibit CD8+ T cell migration by systematically downregulating CXCR3 expression via IL-17A/STAT3 in advanced-stage colorectal cancer patients

Fig 1

Decreased infiltration of CD8+ T cells and increased CXCL10 secretion in tumor tissues from advanced-stage CRC patients. a FACS analysis of CD8+ T cell infiltration in tumor tissues of early- and advanced-stage CRC patients. b CD8 expression was analyzed by immunohistochemistry in tumor tissues; magnification, × 200 (left). Right panel, IRS (0–9) = intensity score (0–3) × percentage score (0–3). c Kaplan-Meier OS curves of CRC patients, presented as high-CD8 or low-CD8 expression groups based on the log-rank statistic test (n = 70). High, IRS > 4; low, IRS ≤ 4. d, e Relative expression levels of effective immune cell-associated chemokines in CRC tumor tissues determined by real-time PCR. f CXCL10 expression was analyzed by immunohistochemistry. g Conditioned media were cultured in the lower chambers of a Transwell plate with or without rhCXCL10. Migrated PBMCs from HDs were collected and assessed via flow cytometry. Each line represented a different HD. h The migratory ability of purified CD8+ T lymphocytes (purity > 90%) from HDs to rhCXCL10 at different concentrations (left). Supernatants of primary tumor tissues were added alone or with CXCL10 (50 ng/mL)-specific neutralizing antibodies as indicated. After incubation, CD8+ T cells alone or cells pretreated with anti-CXCR3 that migrated into the lower chambers were collected and counted. The migration index was calculated by dividing the number of cells that migrated in the indicated groups by the number that migrated in the control groups (right). *P < 0.05, **P < 0.001, ***P < 0.0001; NS non-significant

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