Fig. 5
IL-17A, predominately secreted from Th17 cells, inhibits recruitment of CD8+ T cells via STAT3. a, b FACS analysis of Th17 cell (IFN-γ-IL-17+CD4+ T cells) expression in PB from CRC patients and HDs (a) as well as early- and advanced-stage CRC patients (b). c The expression of 13 cytokines in supernatants of Th17 cells induced by CD4+ T cells from HD PBMCs were analyzed by multiplex assays. d Enriched Th17 cells were subjected to immunofluorescence for IL-17A (green), CD4 (red), and DAPI (blue). e, f CXCR3+CD8+ T cell expression (e) or migratory ability of CD8+ T cell (f) after treatment with rhIL-17A (20 ng/mL), Stattic (10 nM), and/or the supernatants of enriched Th17 cells (5 × 106) with or without anti-IL-17A (10 ng/mL) for 48 h. *P < 0.05, **P < 0.001, ***P < 0.0001