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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: Th17 cells inhibit CD8+ T cell migration by systematically downregulating CXCR3 expression via IL-17A/STAT3 in advanced-stage colorectal cancer patients

Fig. 6

Blocking the STAT3 pathway rescues CD8+ T cell recruitment inhibited by Th17 cells in vivo. a Experimental scheme for the subcutaneous carcinoma model of BALB/c nude mice. CD8+ T cell were pretreated with rhIL-17A and/or Stattic for 48 h in vitro. Then the pretreated CD8+ T cells were intravenously injected on day 0 and tumors were harvested on day 2. Mice were divided into four groups (n = 5/group) based on CD8+ T cell pretreatment. b Immunohistochemistry of CD8 in tumor tissues; magnification, × 200. c CD8+ T cells were quantified using the ImagePro Plus software (Media Cybernetics) and expressed as the positive cells in 20 high-powered fields imaged by microscopy. Three fields were evaluated in one slice. d Experimental scheme for the subcutaneous carcinoma BALB/c nude mouse model. CD8+ T cells, Th17 cells, or CD8+ T cells + Th17 cells, were intravenously injected on day 0. Stattic was administrated intraperitoneally on day 0–7. Tumors were harvested on day 18. Mice were divided into six groups (n = 5/group). e, f Quantitative photon counting analysis of tumor progression by an in vivo imaging system. gi CD8+ and CXCR3+CD8+ T cell expression in tumors and blood were examined by flow cytometry analysis. j Immunofluorescence of CD8 (red) expression in tumor tissues. Three fields were evaluated in one slice

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