Fig. 2

Fabrication of HCC-focused arrays. a According to the screening results of HuProt arrays, 81 proteins (p ≤ 0.05, FC ≥ 1.2 and positive ratio ≥ 10%) were selected as potential candidates. A total of 100 proteins were printed to fabricate the HCC-focused arrays, including 19 proteins from previous reports. b Six representative HCC-focused arrays testing the same sample exhibited high reproducibility. The diagonal indicates the SNR distribution of the sample, the lower left indicates the bivariate scatter plot with a fitted line, and the upper right indicates the correlation coefficient and the significance (***p < 0.001). c HCC-focused arrays were incubated with samples from one HCC patient, one patient with liver cirrhosis, and one healthy control, respectively. Three-dimension renderings of the signal intensities were shown, indicating that the array worked well