Fig. 1

Expression levels of CD46 and oncolytic effect of BVDV on several hemopoietic cancer cell lines. Representative histogram plots of flow cytometry showed CD46 expression levels on a four HMCLs (JJN3, NCI-H929, MM.1S, and OPM2) and b two T-ALL lines as SKW3-KE37, SUP-T1, two B-ALL lines as NALM-6, RS4;11, and two B cell lymphomas lines as GRANTA-519, RAJI. The graphs represent the CD46 median fluorescence intensity (MFI). The picture shows the presence of BVDV in MM cell lines (c) and in non-MM cell lines (d) evaluated by Nested multiplex PCR after 24 h of BVDV (1 MOI) treatment. GAPDH was used as internal quality control. e The histograms represent the percentage of 7-AAD⁺cells after 24, 48, and 72 h of treatment with BVDV (1 MOI). We reported the mean ± SD percentage of dead cells, as 7-AAD⁺ cells, of four independent experiments on JJN3, MM1.S, OPM2, and NCI-H929 and (f) three independent experiments of non-MM cells (SKW3-KE37, SUP-T1, NALM-6, RS4;11, GRANTA-519, RAJI); p values were calculated by two-tailed Student’s t test. (*p < 0.05, **p < 0.01, ***p < 0.001) (CNT = control, untreated cells)