Fig. 6

BVDV treatment inhibit tumoral growth in MM NOD/SCID mouse model. a Scatter plot represents the tumor mass (mm²) after 4, 6, 10, 13, and 16 days of intratumoral treatment with BVDV (blue dots) or PBS (CNT, violet dots) in mice with palpable plasmacytoma. Data are reported as individual values (plots) and the median range (bars). b Box plot graph reports the volumes of tumor mass collected after mice sacrifice in the untreated control condition and in the BVDV group. Values are reported as median volume and the range. p values were calculated by Mann-Whitney test. c Representative hematoxylin and eosin staining (top) and photographs of removed tumor masses (bottom) from one representative mouse of the control group and one of the BVDV-treated group (original magnification, × 1). d The picture shows the presence of BVDV in all the tumor treated of which was possible the collection after the end of the experiment (n°3), evaluated by Nested multiplex PCR. GAPDH was used as internal quality control. e Hematoxylin and eosin in staining of one tumor from the control group and one tumor from the mice treated with BVDV highlighting the tumor necrosis in the BVDV group (original magnification × 20). f Western blot of active-caspase 3 expression on plasmacytomas lysates obtained from one mouse treated with saline solution (mouse CNT) or one mouse treated with BVDV (mouse BVDV). β-actin was used as loading control and JJN3 treated with high doses of Bor as positive control (Cnt+). The histogram represents the protein bands intensity quantified using ImageJ software reported as arbitrary unit normalized by the loading control