Fig. 2

DT2216 is more potent against TCL but less toxic to platelets than ABT263 in vivo. a Illustration of the experimental design of the MyLa xenograft model. b–d Blood platelet (PLT), white blood cell (WBC), and red blood cell (RBC) counts in mice were measured 1 day after receiving the first dose of vehicle (VEH), ABT263, or DT2216. The data presented are mean ± SEM (n = 8 mice per group at the start of treatment). a and b, p < 0.05 vs. VEH and ABT263, respectively. e Body weight changes in MyLa tumor-bearing mice after the start of treatment with VEH, ABT263, or DT2216 as shown in a. Data are presented as mean ± SEM (n = 8 mice per group at the start of treatment). f Changes in tumor volume over time after the start of treatment as shown in a. Data are presented as mean ± SEM (n = 8 mice per group at the start of treatment). g Kaplan–Meier survival curve showing the survival of mice after MyLa engraftment. Median survival time within each treatment group is presented along with statistical analysis results (n = 8 mice in each group at the start of treatment). *p < 0.05, **p < 0.01, and ***p < 0.001 in indicated comparisons. h Immunoblot analysis of Bcl-xL, Bcl-2, Mcl-1, Caspase-3, and PARP in primary tumors (n = 4 mice in each group). Proteins were extracted from the primary tumors after euthanization when mice reached the experimental endpoint as described in the “Materials and methods” section. i–m Quantification of Bcl-xL, Bcl-2, Mcl-1, Caspase-3, and PARP as shown in h. a and b, p < 0.05 vs. VEH and ABT263, respectively. Data are presented as mean ± SEM (n = 4 mice per group). cCaspase-3, cleaved or activated caspase-3; PARP, poly ADP ribose polymerase; cPARP, cleaved PARP