Fig. 5

DT2216 more effectively reduces TCL burdens of TCL PDX mice when combined with ABT199.a Illustration of the experimental design of the second study on DFTL-28776 PDX xenograft model. A group of age-matched female NOD/SCID control (CTL) mice without DFTL-28776 engraftment or any other treatments were included for comparative purposes. b Changes in blood DFTL-28776 PDX cell burden over time after the treatment as shown in a. The data presented are mean ± SEM (n = 6 mice per each group). c Spleen DFTL-28776 PDX cell burden in the PDX xenograft mice after treatment as shown in a. The data presented are mean ± SEM (n = 6 mice per each group). a, b, c, and d, p < 0.05 vs. VEH, ABT263, DT2216, and ABT199, respectively. d Bone marrow DFTL-28776 PDX cell burden in the DFTL-28776 PDX xenograft mice after treatment as shown in a. The data presented are mean ± SEM (n = 6 mice per each group). a, b, c, and d, p < 0.05 vs. VEH, ABT263, DT2216, and ABT199, respectively. e Liver images of DFTL-28776 PDX cell-engrafted mice. f Liver weight in DFTL-28776 PDX xenograft mice after treatment as shown in a. The data presented are mean ± SEM (n = 5 for the CTL group and n = 6 mice for other groups). a, b, c, d, and e, p < 0.05 vs. CTL, VEH, ABT263, DT2216, and ABT199, respectively. g Spleen images of DFTL-28776 PDX cell engrafted mice. h Spleen weight in DFTL-28776 PDX xenograft mice after treatment as shown in a. The data presented are mean ± SEM (n = 5 for the CTL group and n = 6 mice for other groups). a, b, c, d, and e, p < 0.05 vs. CTL, VEH, ABT263, DT2216, and ABT199, respectively. i Hematoxylin & eosin (HE) staining of the spleens and livers from DFTL-28776 PDX cell-engrafted mice. j Human CD45 (hCD45) staining of the spleens and livers from DFTL-28776 PDX cell-engrafted mice. Red color, hCD45 staining with vectastain; blue color, nuclear staining with DAPI