Fig. 3.

Cell proliferation induced by ETV4 is due to the modulation of p21 and p27. a Relative expression levels of murine Cdkn1a mRNA measured by qRT-PCR in the prostate lobes (VP, DLP, and AP) of ETV4 transgenic mice (lines ETV4 A and ETV4 B) normalized to wild-type mice (WT). The analysis has been performed in triplicate in the indicated number of mice (n). Average and standard error values are shown. *P ≤ 0.05, **P ≤ 0.01. b Representative western blots of p21 and p27 proteins in the 3 prostate lobes of wild-type (WT) and of ETV4 transgenic mice (lines ETV4 A and ETV4 B); beta-actin was used as loading control. The relative densitometric quantifications are shown for each lane. c Bar diagram of murine p21 and p27 protein levels measured by western blot analysis in the prostate lobes (VP, DLP, and AP) of ETV4 transgenic mice (ETV4) normalized to wild-type mice (WT). Average and standard error values are shown; n: number of mice. *P ≤ 0.05, **P ≤ 0.01. d Representative microphotograps of p21 (on the left) and p27 (on the right) expression showed by immunohistochemistry analysis in the anterior prostate of WT and ETV4 mice (magnification × 200)