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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: Tie2-mediated vascular remodeling by ferritin-based protein C nanoparticles confers antitumor and anti-metastatic activities

Fig. 4

TFG and TFMG enhanced immune responses in LLC tumors and spontaneous MMTV-PyMT breast tumors. a, b Fluorescence microscopic images of tumor sections harvested from the LLC tumor-bearing mice (a) and from the MMTV-PyMT tumor-bearing mice (b) to illustrate CD4+ and CD8+ T lymphocytes. Frozen tumor sections from vehicle (PBS), TFG, or TFMG-treated groups were immunostained using PE-conjugated CD4 and Alexa Fluor 647-conjugated CD8a antibodies (n = 3). The scale bars: 50 μm. c The percentages of CD4+ T cells and CD8+ T cells population in the mouse tumor model with/without TFG or TFMG treatment (n = 3 per group). *P < 0.05 vs. the vehicle control (PBS). The data are presented as the mean ± SD. d TUNEL assay was performed with LLC and MMTV-PyMT tumor tissues in central and peripheral regions of the tissues, quantified for positive fluorescent cells, and graphed. DAPI staining for nucleus was performed (n = 3). ****P < 0.0001, *P < 0.05. The scale bars: 50 μM. e, f Macrophage infiltration into the LLC tumor (e) and into the MMTV-PyMT tumor (f) were evaluated. The number of infiltrating macrophages was determined by counting CD68+/arginase-1 (Arg-1)+ M2 macrophages (a) or CD68+/induced nitric oxide synthase (iNOS)+ M1 macrophages (b) in the tumor area. Quantitation of the CD68+, iNOS+, and Arg-1+ areas in three random microscopic fields in mice (n = 3) per group was performed using Image J software. Scale bars: 50 μm. Data information: Data are presented as the mean ± SD. Significant enrichment: *P < 0.05, **P < 0.01; ***P < 0.001; (Sidak’s multiple comparisons test)

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