Fig. 3
From: Discovery of a dual inhibitor of NQO1 and GSTP1 for treating glioblastoma
MNPC inhibits NQO1 and GSTP1 and mediates the oxidative stresses in U87MG/EGFRvIII cells. a MNPC inhibits U87MG/EGFRvIII cell proliferation in a dose-dependent manner. Cell proliferation of U87MG/EGFRvIII cells treated with MNPC at the dose of 0, 0.5, 1, 2 and 5 μM for 5 days, and MTT assay was conducted. b NQO1 and c GSTP1 enzyme activity inhibition by MNPC. d The ROS levels, e LDH levels, f Carbonyl Expression and g GSH/GSSG levels in U87MG/EGFRvIII cells after treatment with MNPC. h Western blot analysis of U87MG/EGFRvIII cells treated with different concentrations of MNPC. i Effect of MNPC on apoptosis confirmed by TUNEL assay. The TUNEL assay was carried out by TUNEL Assay Kit. The images of TUNEL positive cells were captured by a confocal microscope. Scale Bar: 20 μm. The cells were treated with 0, 0.5, 1, 2 and 5 μM MNPC for 48 h. j The bar graph represents the quantification of TUNEL assay. Data are means ± SEM (*p < 0.05; **p < 0.01; ***p < 0.001, n = 3)