Fig. 2

GSDEM deletion mitigates DSS-induced colitis. Gsdme^−/− (KO) mice and wild-type (WT) littermate controls were treated with 7-day DSS to induce acute colitis (n = 8 per group); then, body weight change (a) and disease activity index (b) were measured daily. c Immunoblot analyses of GSDME and GSDME-NT in the colonic mucosa of DSS mice. The full length of GSDME (GSDME-FL) is shown in 56 kDa and GSDME-NT in 37 kDa. d, e Representative HE-stained sections of middle colon tissues collected at day 7. Histopathology change reflected by semiquantitative scores (Scale bars: 1 mm). All data shown are representative of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001