Fig. 5
HMGB1 activates the ERK1/2 signaling pathway. a, b Wild-type (WT) mice were induced CAC with AOM/DSS as described above, and in this process, they were intraperitoneally injected with or without neutralizing anti-HMGB1 antibody (HMGB1-ab) at days 1, 3, 5 during DSS treatment. Tumor tissues in the colon were excised at day 84 of the experimental procedure, and whole tissue extracts from the tumors were prepared and analyzed for the phosphorylation of ERK1/2, JNK and P38 by western blotting. a The representative images. b Quantitative analyses of proteins. Data are shown as means ± SEM from five mice in each group. Data shown are representative of three independent experiments. c, d CT26 cells (1 × 105 cells/well) were seeded in 6-well plates. After 24 h, the cells were incubated for 1 h with or without U0126 (10 μM) before stimulation with HMGB1 (1 μg/ml) for 12 h; then, whole cell extracts were analyzed for the phosphorylation of ERK1/2 by western blotting. c The representative images. d Quantitative analyses of proteins. Data are shown as means ± SEM from three independent experiments. NS not significant; ***P < 0.001