Fig. 1

Aberrant expression of miR-934 significantly correlates with liver metastasis and poor prognosis of CRC patients. a Volcano plot comparing the miRNA-Seq data between stage I and stage IV CRC patients from the TCGA CC dataset. Each dot represents a microRNA. Dots in red represent microRNAs that are significantly upregulated in stage IV CRC patients (p < 0.05 based on DESeq). b miR-934 expression in stage I and stage IV CRC patients. Each dot represents a sample. c, d Expression levels of miR-934 in tissues and serum of two groups of CRC patients classified on the basis of the presence (n = 50) or absence (n = 60) of liver metastasis. e Representative in situ hybridization assay for the detection of miR-934 in normal mucosa (score = 0), CRC tissue without liver metastasis (score = 1), and CRC tissue with liver metastasis (score = 3). f, g Kaplan–Meier survival analysis with the log-rank test was used to determine the association of miR-934 with OS (e) and DFS (f) in 308 CRC patients (*p < 0.05; **p < 0.01; ***p < 0.001)