Fig. 1

CSF1-Fc treatment induced significant expansion of BM resident macrophages. a Schematic of CSF1-Fc treatment regimen in C57BL/6 mice (D, day; S.C., subcutaneous). Tissues were harvested at 7 (D7) and 14 days (D14) post-first CSF1-Fc injection. b F4/80 immunohistochemistry (brown) in femoral BM sections of mice treated with saline (left panel) or CSF1-Fc at D7 (middle panel) and D14 (right panel). Closed arrows indicate perivascular macrophages, and arrowheads highlight endosteal macrophages. Sections were counterstained with hematoxylin (blue) and taken at 600X magnification. Scale bar = 20 µm. c Quantification of percent area of F4/80 staining in the femur of saline (blue circles, pooled D7 and D14 samples) and CSF1-Fc-treated mice at D7 (red squares) and D14 (green triangles) post-first injection. d–g Flow cytometry analysis to determine absolute number of cells per femur of d F4/80⁺Ly6G^negVCAM^negCD115⁺CD11b⁺ monocytes, e CD11b⁺Ly6G⁺ granulocytes, f CD11b^negCD3^negB220⁺ B cells and g CD11b^negB220^negCD3⁺ T cells in BM of saline controls or CSF1-Fc-treated mice. Flow cytometry representative raw data and gating provided in Additional file 1: Figs. S1 and S2. Each data point represents a separate mouse, and bars are mean ± SD. Statistical analysis was performed using one-way ANOVA Tukey’s multiple comparison test where ****p < 0.0001