Fig. 2

CSF1-Fc treatment transiently disrupts splenic architecture and altered red pulp macrophage phenotype. a Immunofluorescence labelling of F4/80 (blue), CD169 (red) and B220 (green) expression in spleen sections of mice treated with saline (left panel) or CSF1-Fc and assessed at D7 (middle panel) and D14 (right panel). Magnification = 100×; scale bar = 100 µm. Inset magnification = 600×; scale bar = 20 µm. b Spleen weights in saline controls (blue circles) or CSF1-Fc-treated mice at the D7 (red squares) and D14 (green triangles) time points. c–e Morphometric analysis of percent areas of c F4/80 immunolabelling, d CD169 immunolabelling and e F4/80 area co-labelled with CD169 per mm² of tissue. f–i Flow cytometry analysis of total number of cells per mg of spleen of f F4/80⁺Ly6G^negVCAM^negCD115⁺CD11b⁺ monocytes (Mo), g CD11b⁺Ly6G⁺ granulocytes (Grans), h CD11b^negCD3^negB220⁺ B cells and i CD11b^negB220^negCD3⁺ T cells in saline control or CSF1-Fc-treated mice at both time points. Each data point represents a separate mouse, and bars are mean ± SD. Statistical analysis was performed using one-way ANOVA Tukey’s multiple comparison test where ****p < 0.0001, **p < 0.01 and *p < 0.05, n = 3 to 11 mice/group. Kolmogorov–Smirnov test revealed non-normality for data in graph f, therefore dictating use of a Mann–Whitney U test. Data for the saline control samples from D7 and D14 were pooled together in the graphical representations