Fig. 4

CSF1-Fc treatment is associated with a delayed increase in HSC and MPP in BM and spleen. a Flow cytometry analysis to determine the number of HSC (top left), MPP (top right) and HPC (bottom left) in BM, spleen or liver of C57BL/6 mice treated with saline (blue circles) or CSF1-Fc at D7 (red squares) or D14 (green triangles) post-first CSF1-Fc injection. Quantification of CFU in the BM (assay using single femur only) and spleen of saline or CSF1-Fc-treated mice at both time points (bottom right). b Number of CMP (top left), GMP (top right), MEP (bottom left) and CLP (bottom right) cells in BM, spleen and liver of C57BL/6 mice treated with saline (blue dots) or CSF1-Fc at D14 (green triangles) post-first CSF1-Fc injection. Population gating strategies are exemplified in Additional file 1: Fig. S7. Each data point represents a separate mouse, and bars are mean ± SD. Statistical analysis was performed using one-way ANOVA Tukey’s multiple comparison test where ****p < 0.0001, ***p < 0.0005, **p < 0.01 and *p < 0.05, n = 4 to 11 mice/group. Kolmogorov–Smirnov test revealed non-normality for liver data in graphs in (a), therefore dictating use of a Mann–Whitney U test. Data for the saline control samples from D7 and D14 time points were pooled together in the graphical representations