Fig. 1

Neutrophils labelled by CellTags were Reprogrammed into MPP. a t-SNE visualization of 12,521 cells labelled by CellTags on day 1 (left) and 13,547 CellTags-labelled cells with HSPC program on day 7. “High” and “Low” indicate the mean expression levels of HSPC gene sets. b Sankey diagram showed the reprogramming efficiency from initial hematopoietic cell lineages into induced cells with HSPC program. c PCA analysis of primary HSC, initial neutrophils and the neutrophil derived cells revealing cell fate transition of chemical cocktail-induced reprogramming. d Clustering heatmap of 389 top DEGs of the cells shown in (c) (left) and GO analysis of the three gene sets (right). e Neutrophil-derived cells were cultured on feeder cells then analyzed by FACS for lymphoid cell markers. f Neutrophil-derived cells (CD45.1) were transplanted into sublethal irradiated CD45.2 mice. Donor cells in recipient peripheral blood were monitored by FACS analysis