Fig. 2

Construction of neutrophil reprogramming trajectory by the chemical cocktail induction. a Clustering heatmap of 1600 top DEGs of the neutrophil derived cells by chemical cocktail induction on day 7. b Topological map of neutrophil reprogramming. FA visualization of 1468 cells induced from neutrophils was identified by Leiden clustering algorithm in Scanpy. The relationship of clusters was calculated by PAGA algorithm (right). c Mean expression of specific genes for each cluster in PAGA layout. d FACS analysis of neutrophil markers with different maturation stages on day 8 after chemical cocktail induction (left). Statistical analysis of cell percentage (right). e Quantification of cell percentage of neutrophils with different maturation stages on different days after chemical cocktail induction. f Wright-Giemsa staining of cells on different days. g Schematic model of the main trajectory map of chemical cocktail-induced neutrophil reprogramming