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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Abnormally activated OPN/integrin αVβ3/FAK signalling is responsible for EGFR-TKI resistance in EGFR mutant non-small-cell lung cancer

Fig. 1

OPN is involved in acquired EGFR-TKI resistance in NSCLC. a Expression of soluble receptors and related proteins was examined by proteome profiler array analysis in HCC827, PC9, HCC827GR and PC9 GR cells. b, c The expression level of OPN was determined by quantitative real-time PCR and western blot analysis. d Expression of secreted OPN (sOPN) was verified by ELISA in HCC827GR and PC9GR cells. e, f qRT-PCR and ELISA were used to detect expression of OPN in PC9GR cells after transfection with si-OPN. g The sensitivity of PC9GR cells transfected with si-OPN or si-NC to gefitinib was determined by CCK-8 assays. h Western blot analysis was used to detect expression of p-AKT and p-ERK in HCC827GR cells transfected with si-OPN or si-NC (**P < 0.01; ***P < 0.001)

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