Fig. 9

GPX4 regulated apoptosis through upregulation of EGR1 in TNBC cells. a The expression analysis of EGR1 in normal individuals and breast cancer tissues by UALCAN. b The expression analysis of EGR1 in breast cancer tissue with different stages and normal breast tissue by UALCAN. c The IHC analysis of EGR1 expression in breast cancer tissue and normal breast tissue in the human protein atlas datasets. d Western blot analysis of apoptosis related proteins of mitochondrial pathway after EGR1 knockdown. e Western blot analysis of EGR1 expression after GPX4 knockdown in MDA-MB-231 cells. f Western blot analysis of EGR1 expression after GPX4 knockdown in SUM159 cells. g Western blot analysis of EGR1 expression after DMOCPTL treatment for 24 h or 48 h in MDA-MB-231 cells (left) and SUM159 cells (right). h The representative images of cell apoptosis in MDA-MB-231 cells after EGR1 knockdown with the treatment of CCCP or DMOCPTL (left). The statistical results of cell apoptosis in MDA-MB-231 cells after EGR1 knockdown with the treatment of CCCP or DMOCPTL (right). Analysis results represented mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001