Skip to main content
Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: HOMER3 facilitates growth factor-mediated β-Catenin tyrosine phosphorylation and activation to promote metastasis in triple negative breast cancer

Fig. 2

HOMER3 promotes growth factor-induced β-Catenin tyrosine phosphorylation and activation. a Gene set enrichment analysis (GSEA) of TCGA breast cancer stratified by HOMER3 expression (upper tertile vs. lower two tertiles) using the C6 signature set (oncogenic signatures). The most 15 significantly correlated signatures (positively and negatively) were shown. FDR q, false discovery rate q value; NES, normalized enrichment score. b Enrichment of β-Catenin-upregulated gene signature (BCAT_BILD_ET_AL_UP) and β-Catenin-repressed gene signature (BCAT_BILD_ET_AL_DN) was indicated. c Normalized luciferase activities of specific TOP-Flash over non-specific FOP-Flash relative renilla luciferase units (RLU) in MDA-MB-231 cells treated with PBS, Wnt3A, EGF or TGFα. ns, not significant. d Endogenous levels of HOMER3 in non-TNBC and TNBC cell lines. For tyrosine phosphorylation examination, cell lysates from indicated cell lines were subjected for immunoprecipitation of β-Catenin, followed by western blot analysis with p-Tyr-specific antibody and total β-Catenin antibody. e Tyr phosphorylation levels of β-Catenin in control and HOMER3 silencing MDA-MB-231 and SUM159PT cells treated with PBS, EGF or TGFα. f Western blot analysis of HOMER3, p-β-Catenin-Y333, p-β-Catenin-S33/37/T41 and total β-Catenin in indicated MDA-MB-231 cells. g Nuclear fractions were extracted and subjected for examination of β-Catenin protein. P84 was used as nuclear loading control. h Fluorescence immunostaining of β-Catenin expression in control and HOMER3 silencing MDA-MB-231 cells with PBS or EGF treatment. i Relative expression levels of two typical β-Catenin downstream genes c-Myc and MMP-7 were examined by real-time PCR. j Enrichment of β-Catenin and TCF-4 on c-Myc and MMP-7 promoters was determined by ChIP assays. *P < 0.05; **P < 0.01; ***P < 0.001

Back to article page