Fig. 1

Expression of B7-H3 in human tissues. a IHC of FFPE tissues was used to evaluate B7-H3 expression in various human tumors. The sale bar represents 20 μm. b Western blot analysis of B7-H3 in tumor (T) and paracancerous (P) tissues from four NSCLC patients. The tissue lysates were probed with the goat anti-B7-H3 antibody. The levels of GAPDH were utilized as loading control. c Differential expression of B7-H3 in normal lung tissues, lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) in different stages was analyzed using RNA-seq datasets from TCGA. Significant differences were calculated using Student’ t test (*p < 0.05, **p < 0.01, ***p < 0.001)