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Fig. 7 | Journal of Hematology & Oncology

Fig. 7

From: Targeting B7-H3 via chimeric antigen receptor T cells and bispecific killer cell engagers augments antitumor response of cytotoxic lymphocytes

Fig. 7

Anti-B7-H3 blockade influences on glucose metabolism and intracellular ROS in A549 tumor cells. af Extracellular acidification rate and oxygen consumption rate measurements using the seahorse extracellular flux analyzer. After A549 cells were treated with either anti-B7-H3 or control antibodies for 24 h, ECAR and OCAR were examined using the Mito Stress Cell and Glycolysis Stress assays. ECAR (a, e) and OCR (b, f) metabolic profiles are shown. c Nonglycolytic acidification and glycolytic capacity were derived from Glycolysis Stress assay results. d Nonmitochondrial respiration, ATP production and basal respiration were derived from Mito Stress Cell results. g Intracellular ROS measurement in tumor cells. A549 cells were labeled with 2,7-dichlorofluorescin diacetate as a probe and treated with either anti-B7-H3 or control antibodies. Intracellular ROS signals were measured using flow cytometry. Data from two independent experiments are presented as the mean ± standard deviation. (*p < 0.05, **p < 0.01, ***p < 0.001). The p values were analyzed using a Student’ t test

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