Fig. 1

Increased NOD2 expression correlates with poor prognosis of HCC. a Relative NOD2 mRNA expression in 80 paired HCC (T) and adjacent non-tumor tissues (ANT) from Zhongshan Hospital HCC2 (ZHH2). b NOD2 mRNA expression in HCC cohorts from TCGA and GEO (GSE124535 and GSE121248) databases. c Representative expression pattern (left) and relative intensity (right) of NOD2 protein in 30 paired HCC and ANT tissues from ZHH2. d Representative immunohistochemistry images of NOD2 in HCC and ANT tissues. e Immunohistochemistry staining of NOD2 (e1) and p-RIP2 (e2) in 141 paired HCC and ANT tissues from ZHH1 (n = 141). f Kaplan–Meier analysis for OS and TTR according to NOD2 expression in HCC patients from ZHH1. g Kaplan–Meier analysis for OS in TCGA HCC cohorts. h Positive correlation between NOD2 scores and p-RIP2 scores in HCC samples from ZHH1, n = 141; Pearson correlation analysis. Data were shown as mean ± SD, and significance was determined using paired (c, e), and unpaired Student’s t test (b). ** P < 0.01. Scale bar, 50 μm