Fig. 3

Hepatic NOD2 deficiency attenuates DEN/CCl₄-induced liver inflammatory responses. a, b Hierarchical clustering (a) and KEGG pathway analysis (b) of up- and downregulated genes in livers from Nod2^f/f and Nod2^△hep mice treated with DEN/CCl₄ for 4 months. c Relative mRNA expression of IL-6, TNF-α, IL-1β and IFN-γ in livers of Nod2^f/f and Nod2^△hep mice treated with DEN/CCl₄ for the indicated time points, n = 6. d Western blots analysis of NF-κB (p65), JAK2/STAT3 and MAPK (ERK, JNK, P38) pathways in livers of Nod2^f/f and Nod2^△hep mice treated with DEN/CCl₄ for the indicated time points. e Representative immunohistochemistry images and quantification of F4/80 in livers of Nod2^f/f and Nod2^△hep mice treated with DEN/CCl₄ for the indicated time points, n = 5. Scale bar, 50 μm. Data were shown as mean ± SD, and significance was determined using unpaired Student’s t test (c–e). *P < 0.05, **P < 0.01