Fig. 1

CXCL12γ expression by BMSCs. a CXCL12γ expression in normal BM, MM patient BM, as determined by immunohistochemical staining using the CXCL12γ isoform-specific mAb 6E9 (original magnification 200×). Arrowheads indicate CXCL12γ expression cells. b (left) CXCL12γ and (right) CXCL12α mRNA expression in the primary BMSC (pBMSC) samples, the human BMSC lines HS27a and HS5, the HBMEC lines 4LHBMEC and HBMEC 60, and the HMCLs XG1, MM1.S and L363, analyzed by qPCR and normalized to the housekeeping gene RPLPO. The mean ± SD of three independent experiments in triplicate is shown. C Cell surface CXCL12γ expression on two primary BMSCs, BMSC line HS5, and the HMCL XG1, as determined by flow cytometry using mAb 6E9