Fig. 2

HSPGs retain CXCL12γ on the cell surface of BMSCs. a HSPG expression on the BMSC line HS5 and on primary BMSC samples, as determined by flow cytometry using mAb 10E4 against HSPG. b (left) HSPG cell surface expression on HS5 cells transduced with either CRISPR sgEXT1 or CRISPR empty vector control; (right) CXCL12γ cell surface expression on HS5 cells transduced with either CRISPR sgEXT1 or CRISPR empty vector control. c (upper panel) HSPG cell surface expression on primary BMSCs treated with heparitinase or control buffer; (lower panel) CXCL12γ cell surface expression on primary BMSCs treated with heparitinase or control buffer. d CXCL12γ cell surface binding on XG1-WT (wild-type) or XG1-EXT1KO (knockout) cells after pre-incubation of the cells with 1 µg/ml recombinant CXCL12γ. e Exogenous cell surface and intracellular CXCL12γ and CXCL12α expression. HS5-WT and HS5-EXT1KO cells were transduced with (left) pBABE-CXCL12γ-C9 or (right) pBABE-CXCL12α-C9. Intracellular and cell surface expression of (left) CXCL12γ-C9 and (right) CXCL12α-C9 was detected with an anti-C9 antibody