Fig. 5

CXCL12γ retained by membrane-bound HSPGs promotes adhesion of MM cells to BMSCs. a Adhesion of the HMCLs XG1 and MM1.S to the BMSC lines HS5 and HS27a and the HBMEC lines 4LHBMEC and HMMEC-60. Adhesion to 4LHBMEC is normalized to one. Mean ± SD of three independent experiments in triplicate is shown. b Adhesion of XG1 and MM1.S cells to HS5-WT and two independent CXCL12γKO clones. The mean ± SD of three independent experiments in triplicate is shown. Adhesion to HS5-WT is normalized to one. ***, P ≤ 0.001 using one-way ANOVA. c Adhesion of XG1 and MM1.S cells to HS5-WT or HS5-EXT1KO cells. Mean ± SD of three independent experiments in triplicate is shown. Adhesion to HS5-WT is normalized to one. *P ≤ 0.05; **P ≤ 0.01 using unpaired Student’s t-test. d Adhesion of primary MM cells from two patients to HS5-WT, HS5-CXCL12γKO, or HS5-EXT1KO cells. A representative plot for two independent experiments performed in triplicate is shown. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 using one-way ANOVA. e Adhesion of XG1 and MM1.S cells to HS5-WT, HS5-CXCL12γKO, or HS5-EXT1KO cells transduced with either pBABE-empty or pBABE-CXCL12γ vector. Mean ± SD of three independent experiments in triplicate is shown. **P ≤ 0.01; ***P ≤ 0.001 using one-way ANOVA