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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Correction to: Tumor-derived exosomal miR-934 induces macrophage M2 polarization to promote liver metastasis of colorectal cancer

Fig. 2

miR-934 is encapsulated within CRC cell-derived exosomes. a qPCR analysis of the expression levels of miR-934 in the culture medium (CM) of seven different CRC cell lines and one normal colon cell line. b, c qPCR analysis of the expression levels of miR-934 in the nucleus, cytoplasm, and culture medium (CM) of the HCT-8 and LoVo cell lines. These cell lines showed relatively higher miR-934 expression than other cell lines. d qPCR analysis of the expression levels of miR-934 in the HCT-8 and LoVo cell lines treated with control medium or RNase A (2 mg/mL) alone or in combination with Triton X-100 (0.1%), for 0.5 h. e, f Phenotype analysis of exosomes derived from HCT-8, HT29, LoVo, and Caco-2 cells using electron microscopy (e) and Nano Sight nanoparticle tracking analysis (f). g Western blot analysis was performed to detect typical exosomal biomarkers (TSG101, CD9, and ALIX) in exosomes derived from the above four CRC cell lines. h, i qPCR analysis of miR-934 expression in the CM of HCT-8/LoVo cells depleted of exosomes by GW4869 (an inhibitor of exosome secretion) (h) or by ultracentrifugation (i). j qPCR analysis of the expression levels of miR-934 in HT-29/HCT-8/LoVo/Caco-2-derived exosomes (*p < 0.05; **p < 0.01; ***p < 0.001)

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