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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: The construction, expression, and enhanced anti-tumor activity of YM101: a bispecific antibody simultaneously targeting TGF-β and PD-L1

Fig. 2

The antagonistic effect of YM101 on TGF-β signaling pathway and epithelial-mesenchymal transition in cancer cells. a, b Smad luciferase reporter assay to measure the effect of YM101 on canonical TGF-β signaling. In the presence of TGF-β1 (10 ng/ml), Smad-luc-transfected NF639 and 4T1 cells were incubated with YM101 or controls for 24 h. Then, luminescence was detected. c, d Transwell migration and invasion assays to determine the effect of YM101 on TGF-β-regulated cell movement in cancer cells. e Western blotting assays to measure the effect of YM101 on TGF-β-mediated epithelial-mesenchymal transition in cancer cells. After treatment with TGF-β1 (10 ng/ml) plus antibodies for 4 days, epithelial-mesenchymal transition-associated markers were detected. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 denote the significant difference relative to YM101 treatment. α-TGF-β: anti-TGF-β, α-PD-L1: anti-PD-L1

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