Fig. 4

Schematic illustration and structures of LEDGF. a Schematic illustration of LEDGF containing a PWWP (residues 1–93), CR1 (charged region 1, residues 106–142), NLS (nuclear localization signal, residues 146–156), ATH1 and 2 (AT-hooks 1 and 2, residues 178–183 and 191–197), CR2 and CR3 (residues 208–266 and 266–325), and IBD (integrase binding domain, residues 347–429) domain. b, c X-ray structure of LEDGF–Menin–MLL1 complex (PDB: 3U88). The proteins are shown as cartoon with LEDGF in cyan, Menin in orange, and MLL1 in yellow. Overview of the LEDGF–Menin–MLL1 complex in b and a close-up view of the interactions between LEDGF and Menin–MLL1 in c. The key residues involved in the interaction are shown as stick models. Hydrogen bonds are shown as yellow dashed lines. d, e NMR structure of LEDGF–MLL1 complex (PDB: 2MTN) and LEDGF PWWP (PDB: 2M16). LEDGF–MLL1 complex with LEDGF in cyan and MLL1 in yellow in d and a close-up view of H3K36-Me3 pocket of LEDGF PWWP in e. LEDGF PWWP is presented as cartoon with α-helices in cyan, β-sheets in magenta and loops in brown. The key residues involved in the formation of this pocket are shown as stick models with C atoms in yellow