Fig. 1

Tagln2^−/− BMDCs do not optimally control B16F10 tumor metastasis and growth in mice. a Effect of transgelin-2 expression in T cells for adhesion to B16F10 tumor cells. Empty vector (EV)- or transgelin-2 (TG2)-expressing OTI CD8⁺ T cells were co-incubated with B16F10 in the presence or absence of OVA peptide for 2 h, and conjugates were analyzed by flow cytometry. b Transgelin-2 in T cells showed a minimal effect on B16F10 tumor growth in mice. PBS (none), WT (OTI-T), KO (Tagln2^−/− OTI-T), or transgelin-2-overexpressing T (TG2OE OTI-T) cells were adoptively injected into C57BL/6 mice after OVA⁺B16F10 inoculation. B16F10 tumor weights were measured at day 25 post-implantation (n = 7). c Gross images of 8-day-old OVA⁺B16F10 melanoma after s.c. inoculation (3 × 10⁵) in C57BL/6 WT or Tagln2^−/− mice. Tumor weights and sizes were measured at day 8 post-implantation (n = 7). The survival rates of tumor-bearing mice post-implantation are shown. d Expression of transgelins during GM-CSF-induced differentiation. Freshly isolated BM cells were treated with 20 ng/mL GM-CSF and harvested at the indicated days for western blot. e Expression of transgelins in differentiated DCs after LPS (200 ng/mL) stimulation. f Transgelin-2 expression in DCs from WT or Tagln2^−/− mice. Results are representative of three independent experiments (d–f). g Schematic diagram of solid and metastatic tumor models. h, i Gross images of OVA⁺B16F10 lung metastasis and solid tumors. C57BL/6 mice were injected i.v. with media alone, WT DCs, or Tagln2^−/− DCs. After 7 days, the mice were i.v. (h) or s.c. (i) injected with OVA⁺B16F10 cells. The metastatic nodules (h) and tumor weights and sizes (i) were quantified after 8 days. j The survival rates of tumor-bearing mice post-implantation are shown. Data are representative for nine mice in each group. k, l Tumors from I were dissected, and the populations of infiltrated CD3⁺CD8⁺ T cells (k) and the amount of intracellular IFN-γ in TILs were determined by flow cytometry (l). All data from a–l represent the mean of three experiments ± SEM. *P < 0.05; **P < 0.001; ***P < 0.0001