Fig. 4

Tagln2^−/− DCs do not optimally support T cell activation in vivo. a Schematic diagram of the experimental setup for this figure (left). WT or Tagln2^−/− DCs were pulsed with pOVA (323–339), stained with CMFDA-green, and injected into the footpad of OTII recipient mice. A representative photograph of excised popliteal LNs (right). Scale bar, 1 μm. b Flow cytometric plots show activation of migrating DCs in vivo at 24 h post-injection. DCs were isolated from the popliteal LNs of recipient mice and stained with anti-CD40 or CD80. The percent of CD11c⁺ cells in the draining LNs is presented as a bar graph. c DC-mediated T cell activation in vivo. At 24 h post-injection of DCs, OTII CD4⁺ T cells were isolated from the popliteal LNs of recipient mice and stained with anti-CD69 or CD25. All data represent the mean of three experiments ± SEM. NS, not significant. *P < 0.01